Rapid ester biosynthesis screening reveals a high activity alcohol‐ O ‐acyltransferase (AATase) from tomato fruit
نویسندگان
چکیده
منابع مشابه
Divergence in the enzymatic activities of a tomato and Solanum pennellii alcohol acyltransferase impacts fruit volatile ester composition.
Tomato fruits accumulate a diverse set of volatiles including multiple esters. The content of ester volatiles is relatively low in tomato fruits (Solanum lycopersicum) and far more abundant in the closely related species Solanum pennellii. There are also qualitative variations in ester content between the two species. We have previously shown that high expression of a non-specific esterase is c...
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BACKGROUND A key pathway for ester biosynthesis in yeast is the condensation of an alcohol with acetyl-CoA by alcohol-O-acetyltransferase (AATase). This pathway is also prevalent in fruit, producing short and medium chain volatile esters during ripening. In this work, a series of six AATases from Saccharomyces and non-Saccharomyces yeasts as well as tomato fruit were evaluated with respect to t...
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15 صفحه اولLecithin:cholesterol acyltransferase and cholesteryl ester transfer activity from the isolated perfused rabbit liver.
Lecithin:cholesterol acyltransferase (LCAT) and cholesteryl ester (CE) transfer activity accumulate linearly in the recirculated medium of the isolated perfused rabbit liver. The appearance of both activities in the perfusate was blocked by the addition of 10 microM colchicine, indicating that these two proteins are synthesized and secreted by the liver. CE transfer activity catalyzed the net t...
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ژورنال
عنوان ژورنال: Biotechnology Journal
سال: 2016
ISSN: 1860-6768,1860-7314
DOI: 10.1002/biot.201500406